Thursday, March 19, 2020

When to Use Whom vs. Who

When to Use Whom vs. Who Knowing when to use whom versus who can be difficult for even the most careful writers and speakers. Many writers and grammarians hope the day comes when whom is cast aside and designated by dictionaries as archaic. Indeed, Paul Brians, a professor in the Department of English at Washington State University, says, Whom has been dying an agonizing death for decades. Until the last nail is placed in the coffin, however, it will be helpful to learn when to use whom versus who in various circumstances. How and When to Use Whom Put simply, use whom- which is a pronoun- when it is the object of a sentence. If you can replace the word with her, him, or them for example, use whom. Youll know when to use whom if the pronoun is used in the objective case, or action is being done to the pronoun. Take the sentence: Whom  do you believe? The sentence may sound pretentious, even snobbish. But it is correct because whom is the subject of the infinitive to, as well as the object of the sentence as a whole. Turn the sentence around so that the object is at the end: You were talking to whom? When you replace whom with him, it becomes even clearer: You were talking to him.Were you talking to him? When to Use Who If whom is used for the objective case, who is used for the subjective case- when the pronoun is the subject of the sentence, or the person creating the action. Take the sentence: Who is at the door? The pronoun who is the subject of the sentence. Check this by replacing who with a subjective pronoun, swapping in she or he for who, as in: She is at the door.He is at the door. Who is always used as the subject of a sentence or clause, and whom is always used as an object. Examples In the following sentences, who is correctly used in the subjective case. You can check this by replacing the pronoun who with another subjective pronoun, such as she, he, or you, for example: Who is coming to dinner? (He is coming to dinner?)Who was that masked man? (He was that masked man? or He was the masked man.)Sally is the woman who got the job. (She got the job.) As previously noted, youll know when to use whom if the pronoun is used in the objective case, or action is being done to the pronoun, as in: To Whom It May Concern. (It may concern him.)I dont know from whom the love letter came. (The love letter came from him.)They fought over whom? (They fought over him? or They fought over them?)After whom do I enter the stage? (I enter the stage after him.)Whom did you recommend for the job? (I recommended him for the job.)For Whom the Bell Tolls (The title of this famous Ernest Hemingway novel is saying, The Bell Rings for Him.) Some of these sentences may sound odd, and this is why the word whom will probably disappear from the English language one day. As used in these examples, whom sounds a little awkward, even when its technically correct. How to Remember the Difference The key to understanding when to use whom or who is knowing the difference between  subjective  and  objective  case. Once you can easily identify the subject and the object of a sentence or clause, you will be able to figure out the correct usage of who and whom. For instance, if you want to decide which is correct in this sentence: Who/Whom should I consider as a college recommendation? Rearrange the sentence so that it will make sense using him or he. Youll come up with the following choices: I should consider him for the college recommendation.I should consider he for the college recommendation. The pronoun him is clearly better. Therefore, the correct word in the sentence above will be whom. Remember this simple trick, and youll always know when to use whom and when to use who.

Tuesday, March 3, 2020

Sample - User Manual for Snapchat

Sample - User Manual for Snapchat This report outlines the initial proposal for the Snapchat user manual. This report should cover some of the broad and basic information that first-time users of the Snapchat application need to know. This report shall also cover the unique stylistic components that the author shall use in his final report- which would be the actual Snapchat user manual. The purpose of this report then is to guide the author in planning and writing what to write, how to write the contents, and where to place them in the actual manual. Needs Assessment The focus of the project would be on the application Snapchat. Snapchat is a social media application that allows users to share videos called snaps. This is, in fact, a new trend in the digital arena because as of the moment it is the process of sharing photos that is most prevalent. What Snapchat is all about, however, is the process of sharing videos. It is important to note, however, that users may also take and share photos, drawings, and texts and then send them to a controlled list of people (i.e. the recipients). Essentially, Snapchat is a messaging tool that has a high level of multimedia sharing capability. Considering all of these features, it can be said that Snapchat is virtually intended to be used (and can actually be used) by everyone; from businessmen to casual and professional photographers and graphic and video artists. Students may also take advantage of the enhanced sharing capability of Snapchat. What is unique with Snapchat, however, is the fact that it enable s the users to specify how long the snaps (i.e. the sent videos and photographs) can be viewed by their recipients and actually stay in the company’s servers- after which the developers of the app claim that it would already be deleted. Additional Research One of the latest features that Snapchat users can enjoy is the Snapchat Lenses. With lenses, users can take snaps using real-time special effects, sounds, and filters. This way, they would not have to edit their snaps. Whenever there are updates, new filters (e.g. rewind, slow-motion, force touch) and effects may get released so it would be best to frequently update the version of Snapchat installed on the smartphone or a tablet computer. Audience Analysis The intended audience for the Snapchat user manual would be all of the potential users of the application. Some of the specific examples include: Business men Professional and casual photographers Professional and casual graphic artists Students and teachers Government agencies, departments, and organizations For-profit and non-profit organizations Researchers The application user manual that will be published shall cater to all the users’ needs. No separate version of the user manual will be released for a particular group or audience. The target audience for the user manual would be those who have basic to intermediate smartphone and internet navigation skills; those who actually own a smart phone or a tablet computer equipped with a decent camera capable of taking pictures and recording videos- because these are the main hardware requirements for an individual to utilize Snapchat. Suggested Table of Contents About the Manual Acknowledgement Table of Contents Glossary Introduction Wiki Section Wiki Contents Basics Intermediates Advanced Using Snapchat How to take a picture How to take a video How to create a text message How to organize a list of recipients How to send texts, pictures, videos How to store your contents Snapchat Terms, Policies, and Conditions How to Install and Uninstall the Application Setting up and Account Troubleshooting Frequently Asked Questions Participating in the Snapchat users’ community and forum Frequently Asked Questions The most basic information about Snapchat shall be placed within a consolidated FAQ section. The purpose of the FAQ section would be to enable the users to get started in using the Snapchat application. This may contain instructions on how to install the application, where the download links may be obtained, and how to set up the application on their device after installation. This can be presented within any section of the user manual. In any case, the most important component would be the content. This section has to be simple and easy to understand. Design Layout The purpose of the Snapchat user manual is to get the users started on how to use the application. The focus of the writing process should then be on the content because it is the most important component that would determine the success of the project. The design process should, however, be not taken for granted. The design and layout should embody the following characteristics: elegance, professionalism, and neatness. The design should be creative but not to the point that it would look untidy and too artistic. After all, this was not intended to be an art project. The design and layout should make it easier for the readers to read and understand all sections of the content; this is where the neatness component should be factored in. The layout should follow the outline of the contents provided earlier where each section should have its own design in order for the readers to understand how each section is independent of each other.

Sunday, February 16, 2020

Global Operation Essay Example | Topics and Well Written Essays - 1000 words

Global Operation - Essay Example Moreover, there may be disagreements between the local community and PPQ (Cateora and Graham 19). This is because PPQ will introduce new employees who will be accompanied by their family members in the host foreign country. The host foreign country will experience strain in its communities and social amenities, for example, schools. This is because the communities and social amenities where PPQ is situated will experience an enhancement in number of individuals largely from PPQ. Also, the host foreign country will experience issues associated with the unfamiliar characteristic of the dealings between employees of PPQ and the existing people of the region where PPQ has decided to establish its stores to sell their products. It is imperative that PPQ anticipates any issues that may have a negative impact on the host foreign country and device ways of dealing with them before it starts its process of expansion. Question 2 International organizations frequently go through abundant cultur al issues when they extend their activities into diverse and new territories. When a company expands to new territories, its employees may have difficulties dealing with clients who are from a diverse and different culture. There is the issue of cultural sensitivity which requires the employees of the international company to accommodate the cultural practices of their clients. In addition, workers of international organizations may face difficulties in overcoming language barriers in the new areas of operation (Cateora and Graham 122). These may pose a severe challenge to the ability of workers providing adequate and sufficient services to the customers. Individuals with an identical value system, religion, beliefs, and language share a similar culture. As a result, this is imparted to every individual in the cultural system. Therefore, workers of international organizations, who are not from the same cultural system as the customers, find it difficult to comprehend the customersâ €™ cultural system, hence affecting the process of interaction. Question 3 Diversity has become an extremely essential subject in the international field. Any knowledgeable businessperson or manager should be aware that the universe is decreasing in size due to opportunities and services facilitated by globalization. Inventions, for example, the internet has made diversity an extremely essential subject in the international field because it has made engaging in business activities in different parts of the world less complicated, and trade is making the most of the opportunities caused by being diverse. In addition, diversity has made a number of financial restraints that were in existence in the past to be eradicated as organizations are attempting to engage in business activities all over and across the universe (Cateora and Graham 159). Also, diversity has become a significant subject in the international field because people are moving from their native lands to look for emp loyment opportunities in other areas. This is evident in areas, for example, Europe and North America where there are individuals of mixed ancestries and races. Therefore, diversity has enable individuals preserve their original identity while being part of different geographic regions. Question 4 There are a number of things that may happen if issues relating to diversity and multiculturalism are not paid attention to in a global organization. One, the international

Sunday, February 2, 2020

Time Capsule Essay Example | Topics and Well Written Essays - 1000 words

Time Capsule - Essay Example The following six tracks selected are as follows. The Baroque era constitutes a significant shift in music style. One of the most notable composers of this era is Johann Sebastian Bach. While there were a great variety of Bach’s works the selection committee considered, Bach’s composition ‘Sonata no 1’ a characteristic example of his work. This track combines violin and harpsichord in a level of instrumental complexity and poignancy that at the time had not yet been experienced in the history of music. While ostensibly non-secular this music seemingly transcends such categorizations as it exhibits pure musical poignancy. In these regards, there is a sort of mysterious quality to the track that places it at a level of great interest and intrigue to listeners. In addition to these sonic qualities the committee considered that Bach has significant historical importance both for his power compositions as well as the tremendous influence he had on future musicians. His influential nature and iconic historical importance factored greatly into the committee’s selection of this composition for the time capsule. Furthermore, Bach’s harpsichord concertos are recognized by scholars as among the first concertos for the keyboard instrument ever written (Bukofzer 2008). Another notable Baroque era composer selected for the time capsule was Jean Baptiste Lully, with his ‘Symphonie’ the selected work. While Bach’s work was slightly subtle, this composition is highly bombastic with patriotic or nationalistic overtones in its upbeat melody; in these regards, it could fit perfectly in a state or crown sponsored event (Bukofzer 2008). There were a number of factors that led to the committee’s decision. In addition to Lully’s seminal historical importance to the Baroque era, this composition has withstood the test of time and its sonorous, yet patriotic qualities are

Saturday, January 25, 2020

Theories of planned behavior: Smoking

Theories of planned behavior: Smoking To examine if the Theory of Planned Behavior (TPB) predict smoking behavior, 35 data sets (N= 267,977) have been synthesized, containing 219 effect sizes between the model variables using a meta-analytic structural equation modeling approach (MASEM). Consistent with the TPBs predictions, 1) smoking behavior was related to smoking intentions (weighted mean r =.30), 2) intentions were based on attitudes (weighted mean r =.16) and subjective norms (weighted mean r =.20). Consistent with TPBs hypotheses, perceived behavioral control was related to smoking intentions (weighted mean r = -.24) and behaviors (weighted mean r =-.20) and it contribute significantly to cigarette consumption. The strength of associations, however, was influenced by studies and participants characteristics. Smoking remains the leading preventable cause of death and disease in western countries. Despite the constant reduction in smoking prevalence among adults over the last 20 years in developed countries, smoking rates have not decreased among young people, and the highest youth smoking rates can be found in Central and Eastern Europe. In an attempt to understand the psychosocial determinants of smoking initiation and maintenance, a variety of social cognitive models have been applied. One of the most influential theories predicting smoking behavior, the Theory of Planned Behavior (TPB) 1has been used both for conducting a wide range of empirical research on smoking behavior antecedents and for designing many theory-based intervention programs to reduce tobacco consumption. An increasing number of empirical studies have examined this model in relation to smoke and the variability of results suggests that a quantitative integration of this literature would prove valuable. Up to the present, various quantitative reviews of the TPB have been performed but centered in other behavioral outcomes, as exercise, 2 condom use 3 and others. Hence, the purpose of this study was to evaluate the success of TPB as a predictor of smoking behavior through meta-analytic structural equation modeling (MASEM), involving the techniques of synthesizing correlation matrices and fitting SEM as suggested by Viswesvaran and Ones. 4 The TPB, an extension of the Theory of Reasoned Action,5 incorporates both social influences and personal factors as predictors, specifying a limited number of psychological variables that can influence a behavior, namely 1) intention; 2) attitude; 3) subjective norm (SN); and 4) perceived behavioral control (PBC). 1 First, subjective norms are conceptualized as the pressure that people perceive from important others to execute a behavior. Second, peoples positive or negative evaluations of their performing a behavior are conceptualized as other predictor of intention (attitudes). Third, PBC represents ones evaluation about the easy or difficulty of adopting the behavior and it is assumed to reflect the obstacles that one encountered in past behavioral performances. Finally, attitudes, SN and PBC are proposed to influence behavior through their influence on intentions, which summarize persons motivation to act in a particular manner and indicate how hard the person is willing to try and how much time and effort he or she is willing to devote in order to perform a behavior. 6 The TPB has been applied through a relevant amount of primary studies and their predictive utility has been proved meta-analytically both for a wide range of behaviors 7, 6 and for specific health risky or health protective behaviors. 3, 2 These previous meta-analyses, however, have neither examined how useful the TPB is to predict smoking behavior, nor the overall structure of the model applied to tobacco consumption. Hence, some concerns remain relating to TPB and its utility to predict smoking behavior that deserves further examination through MASEM. Firstly, a weakness of the SN-intention relation has been found by previous meta-analysis 7 compared with attitude-intention and PBC-intention associations. It has been suggested that this lack of association indicates that intentions are influenced primarily by personal factors 6. In spite of, some primary studies finding strong beta values, ranging from .44 to .62, for attitude on smoking intention such as Hanson, 8 while others founded values near .18 or .19. 9, 10 At the same time, although researchers have theorized about the importance of PBC in this domain, regarding health-risky behaviors, the correlation between PBC and behavior had sometimes been disappointing. 3 One possible explanation is that PBC may not capture actual control. Other is that risky behaviors performed in social contexts may be more determined by risky-conducive circumstances than by personal factors. 11 Moreover, primary studies on smoking behavior have found contrasting results for PBC -behavior, such as r =.55 12 or r =.06. 13 Based on these discrepant findings, we proposed, as a first purpose of this review, to test the strength of relationships between TPB constructs applied to smoking behavior. Secondly, in order to clarify the influence of moderator variables and to provide further explanation for the variability on the effect sizes (ES) between primary studies, some studies and participants characteristics may be taken into account. Ajzen and Fishbein 5 argued that intention and behavior should be measured as close in time as possible to the behavior. In spite of that, primary studies on smoking behavior 14, 15 have found that beta values for intention- behavior association have been maintained during six months (à ¯Ã‚ Ã‚ ¢=.38), nine months (à ¯Ã‚ Ã‚ ¢=.35) and a year (à ¯Ã‚ Ã‚ ¢=.35). Thus, it is important to quantitatively review the moderator effect of time interval on strength of TPB constructs. It has been recognized that culture provides a social context that affects prevalence of certain behaviors. Moreover, some studies have compared results of TPB applied to smoking behavior by using diverse ethnic groups into the USA, such as Hanson, 8 while a great amount of primary studies have expanded their applicability to different cultural contexts. 16, 15, 10 These studies have revealed contradictory results, such as for Puerto-Ricans and non-Hispanic whites, SN was not found as a significant predictor of intention, 8 while it was significant for African-American teenagers, or beta values for SN-behavior ranging from à ¯Ã‚ Ã‚ ¢=.20 for UK samples 17 to à ¯Ã‚ Ã‚ ¢ =.43 for Netherlanders students. 18 Hence, because of cultural differences with respect to the SN-outcomes association, there is a need to meta-analytically examine the moderator effect of culture. Ajzen and Fishbein 5 and Ajzen 19 also recommended scale correspondence of measures for intention to properly predict behavior. However, meta-analysis on TPB applied to exercise behavior have found that only 50% of examined studies had scale correspondence, 20 and that ES was the strongest for the intention-behavior association when studies had scale correspondence. 2 Based on these previous findings, we contend that a thorough examination of moderator effect of scale correspondence on strength of smoking intention and behavior relationships is needed. Research indicates that teenage years are associated with heightened sensitivity to SN 6 and differences have been found in previous meta-analyses between age groups regarding their intention -exercise behavior association. 2 At the same time, only one study has tested gender differences applying TPB to cigarette smoking, 13 founding that the model fitted better among female students. Despite the fact that no consistent evidence has been found relating to the moderator effect of age and gender on the TPB constructs association, we state that an exploratory analysis would be advisable. Thirdly, while previous studies on TPB on smoking behavior had used stepwise regression analyses, more recent ones apply SEM or path-analyses. When all TPB relationships were tested simultaneously, same patterns would change. For instance, after controlling the influence of intention, the PBC- behavior association would turn to negligible values (à ¯Ã‚ Ã‚ ¢=.05), such as Albarracà ­n et al 3 proved for condom use. Moreover, based on the fruitful results of meta-analysis obtained in many research domains, 3, 21, 22, 23, 24, 25 it can be beneficial to use meta-analytic structural equation modeling techniques (MASEM) in testing causal models, such as some authors suggested. 4, 26 Based on these methodological and conceptual issues, the main objective of this meta-analysis was threefold. The first objective was to test the strength of the relationships between the TPB constructs with the smoking behavior. Specifically, we hypothesized: (1) large ES for intention-behavior, PBC-intention, PBC-behavior, and attitude -intention; (2) moderate ES for SN- intention; (3) larger ES for intention-behavior than for PBC-behavior and (4) larger ES for PBC-intention and SN-intention than for attitude-intention. The second purpose was to test the influence of moderator variables on the relationships between the TPB constructs. Specifically, we proposed (5) larger ES for attitude- behavior, PBC- behavior, SN-behavior, and intention-behavior when measures have been taken simultaneously; (6) larger ES when the time interval was shorter; (7) the largest ES for SN-intention and SN- behavior when participants belong to a collectivist culture, coded as Others into the category orig in of the sample; (8) larger ES for attitude- intention, SN-intention, PBC-intention and intention -behavior when constructs have been measured with scale correspondence; and (9) mean age of the sample, percentage of males and year of publication would moderate the relationships among TPB constructs. The third purpose was to test the predictive utility of TPB on smoking behavior through MASEM analyses. Specifically, we hypothesized that: (10) intention and PBC will predict smoking behavior; (11) attitude, PBC, and SN will predict intention and (12) intention will be a stronger predictor of behavior than PBC. Method Literature search In order to locate relevant studies, we conducted a computerized bibliographic search of the PsycInfo, MedLine, ERIC, using the terms smoke, smoking behavior, nicotine, tobacco consumption, and TPB as keywords. We also conducted a manual search of journals that regularly published smoking behavior research. Descendent searches have been conducted based on the references section of retrieved studies specifically previous TPB meta-analyses including multiple behavioral outcomes- and some authors have been contacted to obtain unpublished papers. This processes resulted in 52 studies retrieved in full text to further screening. Inclusion and exclusion criteria A study was considered for this meta-analysis if it met the following inclusion criteria: (1) the study had to report quantitative research on TPB applied to smoking behavior; (2) the study had to report a Pearson correlation coefficient between TPB constructs or data that enable us to calculate ES. Upon closer examination of the remaining 52 studies, a total of 27 studies were included which provided an amount of 35 independent samples (N= 267,977) and 219 ES. A total amount of 25 studies were excluded. Reasons for elimination have been that TPB construct measures were not included (8 studies), i.e.: 27, or that the studies were focused on smoking cessation instead of on smoking behavior (17 studies), i.e.: 28, 29. Only one dissertation has been included and no unpublished papers have been obtained. The studies that focused on smoking cessation have been excluded because the outcome variable in the model-smoking behavior versus smoking cessation-differs substantially. These studies will be used to conduct a separate meta-analysis on smoking cessation. All the included studies are marked with an asterisk in the reference section. Coding of studies The study characteristics coded were: year of publication, origin of the sample, scale correspondence, and time interval between TPB measures. The subject characteristics coded were: the number or participants, mean age of the sample, and gender (as percentage of men in the sample). We consider relevant to code how smoking behavior was assessed (i.e., objective vs. self-report.) but we could find only one study which used objective measures, as CO (carbon monoxide) tests. 30 Following the procedures of Symons and Hausenblas, 2 the time interval between intention and behavior was examined by classifying the studies as: (1) short (less than or equal to six months), (2) medium (greater than six months and less than or equal to one year), (3) large (greater that one year). Regarding scale correspondence, we examined the method section of each study in search of the detailed information. Such as Symons and Hausenblas suggested 2, scale correspondence has been fulfilled when the same magni tude, frequencies or response formats are used to assess the constructs. If intention and behavior were measured exactly with the same items, we considered that scale equivalence was present. If intention was measured with a broader redaction (i.e.: How certain are you that you could resist smoking this term?) while behavior was assessed by a more detailed item (i.e.: How many cigarettes did you smoke per day?), or by asking participants to classify themselves as non-smoker/current-smoker, we considered that scale correspondence has not been fulfilled. In order to ensure accuracy, the studies were coded by two authors independently, reaching an intercoder agreement of 90%. The level of agreement reached was highly satisfactory and inconsistencies were solved by consensus. Some decisions about independence of the samples were taken. If the same study design was carried out in multiple but independent samples (i. e, boys and girls, asthmatic and no-asthmatic students, African-American, Puerto Rican and Non-Hispanic white teenagers) results were entered into the meta-analysis as independent samples. 8, 18, 13 In other cases, only one ES per study has been considered. Data analysis We followed Hedges and Oldkins 31 meta-analytic fixed effects procedures to estimate weighted mean correlations. In these procedures, correlations were converted using Fishers r to z transformations and weighted by N 3, the inverse of which is the variance of z, in analyses. Using Cohens criteria, 32 ES values of .10, .30 and .50 were considered small, moderate and large effects, respectively. Graphical procedures were used to explore the skewness of data. When an extreme value was detected, analyses were carried both including and excluding the outlier. Next, we tested the homogeneity of the ES (Q statistics) and we analyzed the influence of moderator variables using categorical model (ANOVA analogous) and weighted regression analyses (fixed-effect model). One problem in the interpretation of meta-analytic results is the potential bias of the mean ES due to sampling error or to systematic omission of studies that are hard to locate. According to Orwin, 33 the tolerance index of nul l results should be calculated and there must be more than 300 unpublished studies (and not recovered by the meta-analyst) for the results to be annulled. However, this statement should be qualified because the index by categories yields small values in some of these categories. Therefore, we can conclude that publication bias is not very likely to threaten the results severely. MASEM analyses Meta-analytic structural equation modeling, which involves the techniques of synthesizing correlation matrices and fitting SEM, is usually done by applying meta-analytic techniques on a series of correlation matrices to create a pooled correlation matrix, which then can be analyzed using SEM, as suggested Viswesvaran and Ones. 4 However, these procedures have received criticism by Becker (1992) and more recently by Cheung and Chan. 26 Despite some problems, the major advantage of these univariate approaches are their ease of application in applied contexts. Based on these recommendations, we used Viswesvaran and Ones procedure to test the strength of the association among the TPB constructs with smoking behavior. The complete weighted correlation matrix was 5 x 5 and it was submitted to SEM analyses. The predicted model was fitted assuming the harmonic mean (N= 239) as sample size, 4 and it was estimated with unweighted least squares procedures. The proposed model, according to TPB l iterature, had three exogenous latent variables and two endogenous ones, such as depicted Figure 1. Besides chi-square, we reported Goodness of Fit Index (GFI), Adjusted Goodness of Fit Index (AGFI), Normative Fit Index (NFI), and Root Mean Squared Residual (RMR) as fitness indices. It is typically assumed that GFI, AGFI, and NFI >=.90, RMR values

Friday, January 17, 2020

PLATE

The protein fraction from 70 percent saturation of recrystallised ammonium sulphate was found to have the maximum protein content (19.6 mg/g flower) and hence it was selected for further studies and is abbreviated as PAF in the present study. 4.2.1 Characterization of the selected PAF by Native PAGE and SDS PAGE The selected PAF was characterized by column chromatography. This showed a single peak and was further characterized in native PAGE and SDS page. The results are shown in Plate the SDS-PAGE analysis of the ammonium sulphate precipitated protein extract showed 15 different protein bands with good visibility in CBB R250 staining method (Figure). PLATE CHARACTER IZATION OF PFPa BY PAGE AND SDS PAGE A – Standard Protein Markers; B – PAGE; C– SDS PAGE Each fraction showed a number of major and minor bands indicating several proteins. Of the several bands obtained in 70 per cent saturation of ammonium sulphate, the major band alone was eluted. In order to find out the presence of subunits in this band, it was further subjected to SDS PAGE. The results showed one major protein indicating the absence of subunits. The molecular weight of this protein was found to be 99 KD when compared to the standard molecular markers. 4.3. Fifty percent effective dose of selected protein fraction of Plumeria alba The free radical scavenging capacity of PFC was tested by its ability to bleach the stable DPPH. The DPPH (2,2 diphenyl -1- picryl hydrazyl) radical scavenging activity was carried out using different concentrations of PFC (Figure). The radical scavenging activity was found to be dose dependent. Figure Percentage Free Radical Scavenging Activity of Selected protein fraction of Plumeria alba The protein fraction of Plumeria flower extract showed the dose dependent DPPH radical scavenging activity. From the graph, the 50 percent effective concentrations of these were found to be 28 Â µg and 35 Â µg and used in the further studies. Free radicals and their scavenging systems play important role in the healing of normal and delayed types of wounds. The dose response curve of DPPH radical scavenging activity 0 10 20 30 40 50 60 70 80 90 10010 20 30 40 50 60 70 80 90 100 Ascorbic acid Plant sampleDPPH RadicalScavenging 9(%) of the extract and standards showed that at the highest concentration (0.5mg ml-1) the scavenging effect of the methanolic extract reached 9.3% (Afolayan et al., 2008). Shyuret al. (2005) also reported that the scavenging activity for free radicals of 1,1diphyryl-2-picrylhydrazyl (DPPH) has been widely used to evaluate the antioxidant activity of natural products from plants. The antioxidant activities of the leafy vegetables of India were measured in different systems of assays such as DPPH assay, super oxide radical scavenging assay, hydroxyl radical scavenging assay and lipid peroxidation assay and IC50 values were calculated (Dasgupta and De 2007). 4.4. Matrix-Assisted Laser Desorption/Ionization – Time of Flight Mass Spectra (MALDI-TOF MS) MALDI-TOF mass spectra were used for the analysis of peptide mass fingerprinting and MS/MS ion search identification of the in-gel trypsin digested protein fragments (Figure 5), selected peptide masses were submitted to Mascot (http://www.matrixscience.com) for SwissProt databases search. There was no conclusive match in peptide mass fingerprinting, since MS/MS ion search program was selected for further identification. The MS/MS ion search in the NCBIProt database revealed that, WRKY transcription factor WRKY24-like isoform X1 [Juglans regia] (Figure 6-7) with the protein score of 81 (Protein score is 10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 80 are significant (p

Thursday, January 9, 2020

The Decline and Fall of the Roman Empire Essay - 822 Words

The Decline and Fall of The Roman Empire The Roman Empire was a beautiful place ruled by Augustus. The borders of the empire during the Pax Romana measured 10,000 miles and enclosed an area of more than 3 million square miles, that’s about the size of the United States today. The population of the empire during this period was between 70 and 90 million people. The city of Rome itself was home to about one million people. During the third century (A.D. 200-300), problems confronted the Roman Empire. The decline of the empire continued for almost 300 hundred years. The Roman Empire was brought to its downfall because of the way their Social, Political, and Economic systems were working. Historians say that the Roman Empire began to†¦show more content†¦Advanced militarism attracted men looking for victory. The character of the soldiers changed from armed men fighting to defend their homes and families to men signing up to fight as mercenaries. As demand increased for foreign goods, currency flowed out of Rome to foreign countries. Rome and its people no longer believed in their empire, and didn’t care much about family values anymore. The Economic issues were that they had poor harvest. Food was scarce and people needed food so they went after it. There was a disruption of trade by barbarians and pirates on Mediterranean Sea lanes. Since the war was over, there was no more war booty. Gold and silver were drained away to buy things from foreign countries, this led to inflation, a drop in the worth of money and a rise in prices. The Roman road and bridge system fell out of order, causing a hurt in trade. The crushing tax fell all upon the plebian class, who had no money to start with. Everything was quickly spiraling downhill. The Empire’s economy was worsened by its military problems. Tribes of northern barbarians called Goths overran the legions guarding the Danube frontier numerous times. Instead of fighting for patriotism, Roman soldiers now fought for money. The government promised higher cash awards to attract recruits into the army. Emperors started to recruit barbarians because they wouldShow MoreRelatedThe Decline And Fall Of The Roman Empire913 Words   |  4 Pages A consistent measure throughout all history has been that all great empires fall. Many theories have been given to why the empire fell, but Gibbons famous book, The Decline and Fall of the Roman Empire, provides reasoning for what we consider as why Rome fell. Even though there was an inconsistency of leadership, the main reason for the decline of the Roman Empire was due to the size of the empire. Supporting Gibbons reasoning, Rome fell because the immense size was too large to control, leadersRead MoreDecline And Fall Of The Roman Empire1310 Words   |  6 Pagesthat the Roman Empire was the greatest Empire in history. They destroyed their enemies and took their land. They had control of the Mediterranean Sea and all of the trade that went through it. Places like the Pantheon were constructed and laws such as stare decisis were created. The Roman Empire was an empire that many people wanted to live under. Then, like all empires eventually do, it fell. Looking at history, there are nine possible reasons for the decline and fall of the Roman Empire. The firstRead MoreGibbons History of the Decline and Fall of the Roman Empire 1850 Words   |  8 PagesGibbon’s History of the Decline and Fall of the Roman Empire became unpopular with large groups of the British reading public. The abridged edition consecutively presents the stories behind the Empire’s leadership and course of action. Gibbon revivifies the complex and compelling period of the Romans by detailing the prosperous conditions of the empire, the decline, and the aftermath of the fall. At the same time, Gibbon efficiently scrutinizes the declining virtue of the Roman people. Gibbon made anRead MoreEssay on The Fall of the Roman Empire1078 Words   |  5 PagesFall of the Roman Empire Name: Institution: â€Æ' Fall of the Roman Empire Introduction The Roman Empire faced many problems in the third century. Many of these problems came within the empire and other forces that were outside the empire. The only thing that seemed to aid in the holding of this great empire was drastic economic, political, and military reforms, which looked as essential elements that would prevent the collapse of the empire. Large groups of historians come to terms with the idea thatRead MoreThe Fall Of The Roman Empire1419 Words   |  6 PagesThe Roman Empire was a powerful governing body of extensive political and social structures throughout western civilization. How did this empire fall and were internal factories responsible? Slow occurrences in succession to one another led to the fall of the empire rather than one single event. The fall of the Roman Empire was a combination of both internal and external pressures, not just one, leading up to the complete decay of the cities—Rome and Constantinople. However, one could argue how oneRead M oreReasons For The Fall Of The Roman Empire1127 Words   |  5 PagesWhy the Roman Empire Fell The Roman Empire was the most influential and structured government in history.Although they were more advanced they could not avoid the pitfalls that all countries face.Throughout history and even now civilizations can be dependant on these basic needs such as just political leadership, keeping the middle class at bay,economy, and religious morals,but unfortunately the Roman Empire had difficulties maintaining these basic needs. Through my research I have found severalRead MoreThe Decline Of The Imperial Collapse Of Rome And Greece1725 Words   |  7 Pages Unlike that of China, the decline of Rome was much more upsetting having a much larger impact on the surrounding world. The Decline and fall of the Mediterranean and Chinese civilizations was a result of population decrease, weak government, a frail economy, and invasion. The causes of the imperial collapse in Rome and Greece(The Mediterranean region)with the causes of imperial collapse in the East Asia during the period 600 BCE toward 600 CE has had an remarkable achievements but those sameRead MoreThe Fall Of The Roman Empire1316 Words   |  6 Pagescivilization, the Roman Empire was a powerful governing build. Political, economic and social entities advocated for the success of the empire. However, the question still remains, how was it possible that the very things that once made the empire great could be the sole reasons for its decline? There are substantial reasons as to why the empire fell. Constant occurrences in succession from another—whether internal or external—led to the fall rather than one single event. The fall of the Roman Empire was a combinationRead MoreFall of the Roman Empire1288 Words   |  6 PagesRomana was a two hundred year time period where the Romans had peace and prosperity under Augustus. The Roman empire started to decline at the end of the prevail of the last five emperors, Marcus Aurelius in 161-180 A.D. The rulers in the next century had no idea how to deal with the problems the empire was having. There was many reasons to the fall of the Roman Empire but three stood out the most. The preliminary reason was the economy begins to decline. The alternative reasoning was Rome started toRead MoreEssay about The Fall of the Roman Empire1275 Words   |  6 PagesThe Fall of the Roman Empire A reason that leads to Romes inability to remain self-sustaining as an Empire was its lack of technology. Technological advance did not increase at a rate proportional to the increase of the people per square mile. This lead to the inability of the Romans to become self-sustaining. Once again the slave trade was a reason that their technology levels failed to increase. The bulk of work done in the Roman Empire was always carried out by the slaves. This provided